Identification of non-HLA antibodies in ventricular assist device recipients

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Original research

Identification of non-HLA antibodies in ventricular assist device recipients

Sandy von Salisch1Maja-Theresa Dieterlen1Jens Garbade1Sara Klein1Stefan Dhein1Friedrich W. Mohr1Hartmuth B. Bittner2Markus J. Barten1*

1 University Hospital Leipzig, Heart Center, Department for Cardiac Surgery, 04289, Germany, Leipzig, Strümpellstraße 39
2 Florida Hospital Orlando, Department of Cardiothoracic Transplantation and Advanced Cardiac Surgery, 32804, U.S.A., Florida, Orlando, 2415 N Orange Ave Suite 600

* Corresponding author:
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Aims

Recipients of ventricular assist devices (VADR) have a higher incidence to develop antibodies (Abs) against human leukocyte antigens (HLA). Non-HLA antibodies like major histocompatibility complex class I-related chain A (MICA) and autoantibodies against angiotensin type 1 receptor (AT1R) and endothelin receptor A (ETAR) are also implicated in the pathogenesis of acute rejection and allograft vasculopathy. We monitored non-HLA- and HLA-Abs in VADR up to one year after implantation.

Materials and methods

Sera of 56 VADR (54.1±12.8 years old, 50 men) were analyzed for Abs against HLA-, MICA-, AT1R- and ETAR several times over one year after implantation using ELISA and Luminex xMAP technology. Blood transfusions, gender and age were reviewed.

Results

Sera of 56 VADR (54.1±12.8 years old, 50 men) were analyzed for Abs against HLA-, MICA-, AT1R- and ETAR several times over one year after implantation using ELISA and Luminex xMAP technology. Blood transfusions, gender and age were reviewed.

Conclusion

Beside HLA- and MICA-Abs, VADR showed high titres of Abs against AT1R or ETAR, which underlines the necessity for monitoring non-HLA antibodies in VADR prior heart transplantation.

Imprint

Sandy von Salisch, Maja-Theresa Dieterlen, Jens Garbade, Sara Klein, Stefan Dhein, Friedrich W. Mohr, Hartmuth B. Bittner, Markus J. Barten. Identification of non-HLA antibodies in ventricular assist device recipients; Cardiometry; No.3; November 2013; p.82-99; DOI:10.12710/cardiometry.2013.3.8299 Available from: www.cardiometry.net/no3-november-2013/identification-of-non-hla-antibodies

Keywords

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